Qiagen QIAgility, User Manual

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QIAgility Software
5-74 QIAgility User Manual 11/2011
2. Define the total number of reactions to prepare, using
the “Total reactions to prepare (if unused)” option.
3. If extra volume is required, select “Extra (if unused)?”.
Setting up a premade master mix
1. Left-click on a well in the Master Mix Block. The “Mix”
right-hand pane will appear.
2. Select “Use Master Mix?”, and then select “Use pre-
mixed Master Mix?”.
3. Enter a name for the master mix in the “Master Mix
name” field.
4. Enter the “Volume per sample” and the “Number of
times to mix before use”. The corresponding well in the
Master Mix Block will now be colored green.
Loading a saved master mix setup
1. Click on the “Load” button in the “Machine-mixed
Master Mix” panel.
2. Select a file and then click on the “Open” button.
3. The “Reagent Selector” window will appear. The
reagents are listed in the “Reagents” panel in white
boxes.