Qiagen QIAgility, User Manual

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QIAgility Software
5-88 QIAgility User Manual 11/2011
Reagents
(Advanced
reagents View)
Shows a table of all available reagents on
the worktable. By entering a volume in the
column “Volume to use” the given volume
of the selected reagent is added to the
reaction.
Target reaction
plate
Click on the button to open the
“Plate/well Selector” screen. This screen
allows the user to select the target
reaction plate and well.
Sample volume Defines the volume of sample to be
added to the reactions. When “Use
normalization?” is selected, this option
defines the target volume for normalized
samples in the reactions. An initial value is
automatically set when the operation
includes a master mix configured with
default sample volume. A sample volume
of zero is permitted.
Total volume Defines the per-reaction total volume. The
“Total volume” is automatically
calculated, based on the sample, reagent,
and master mix volumes.
Replicates Defines the number of replicates to be
performed for each sample bank.
Number of
wells
Defines the total number of wells. This
number is automatically calculated, based
on the number of samples in the sample
bank and the number of replicates.
Alternate
sample wells
with diluent?
When constructing reactions, this option
instructs the instrument to alternate
sample wells with diluent. When selected,
the operation prepares successive
reactions using a sample from the bank,
then diluent, then the next sample from
the bank, and so on.