Qiagen QIAgility, User Manual

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QIAgility Software
QIAgility User Manual 11/2011 5-49
Reagent A reagent can be combined with other
reagents to make a master mix, or added
individually to a reaction. Reagents are
always dispensed to reaction wells before
samples.
Mix A master mix is a pooled group of
reagents that can be added to a reaction
plate. Master mix is always added to a
reaction first because it normally
constitutes the largest volume of the
reaction.
Reaction A reaction is the destination for the
samples, master mixes, and reagents.
Reactions can be assigned to a sample
bank — this enables a reaction to be a
component of a different reaction. A
reaction that is a component of another
reaction is called an ‘Intermediate’
reaction.
Standard A standard is a dilution series with known
concentrations. Standards are treated like
samples.
Diluent Diluent is used to dilute the master mix,
dilute samples, and create standard
dilution series. It is assumed to be PCR-
grade water. A diluent can only be
assigned to a single well segment. Only 1
diluent tube can be assigned in a run file.
Tip The plate contains a Tip Rack Holder and
tips. Tips must be set to available or
unavailable.
To change the plate function:
1. Right-click on the plate, or the segment of a plate, that is
to be changed. The plate operations menu will appear.