Qiagen QIAgility, User Manual

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Appendix F
F-4 QIAgility User Manual 11/2011
banks are considered to be intermediate reaction wells. By
default, the software does not account for dead volumes of
the intermediate wells when checking for underflow
conditions. In practice this can result in shortages when
aspirating from intermediate reaction wells. When this
function is activated, the software will generate warnings if
aspirates from intermediate wells would cause the well
volume to fall below its dead volume.
By default, this function is not activated.
Advance Z-axis whilst aspirating
When activated a tick is visible. This function modifies the
aspirate operation to reduce contact between liquid and the
exterior of the tip. When activated, aspirates are performed
by advancing the tip downwards into the well while
aspirating, thereby approximately tracking the liquid surface
during the operation.
By default, this function is not activated.
Retract Z-axis whilst dispensing
When activated a tick is visible. This function modifies the
eject operation to reduce contact between liquid and the
exterior of the tip. When activated, dispenses are performed
by retracting the tip upwards from the well while ejecting,
thereby approximately tracking the liquid surface during the
operation.
By default, this function is activated.
Detect insufficient volume on aspirate
When activated a tick is visible. When using the level sensor
during aspirate operations, the software calculates the
volume present in the well based on the position of the liquid
surface, and generates an error warning if the volume
detected is less than the volume to be aspirated. When
activated, this function suppresses this warning, allowing the
operation to proceed regardless.
By default, this function is activated.