Qiagen QIAgility, User Manual

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QIAgility Software
5-136 QIAgility User Manual 11/2011
different concentrations of the probe and with 3 different
concentrations of primer (i.e., a total of 9 reactions). Several
optimizations are possible using the “Optimisation Wizard”.
Note : The user must provide all required concentrations of
all the variables that are to be optimized.
This section outlines the “Optimisation Wizard” screens that
will guide the user through the setup. Instructions are
displayed on each screen.
Note : The “Optimisation Wizard” only provides steps to set
up an optimized matrix, it does not provide steps for
preparation of a master mix.
To set up a PCR reaction using the “Optimisation Wizard”:
1. Select “Wizard/Optimisation Wizard”, or select
“Optimisation Wizard” from the splash screen that
appears when the software is started.
2. The “Optimisation Wizard” will appear.
3. Select option “1”, “2”, or “3”. These options define the
number of variables to be optimized.
4. Click on the “Next” button to continue.