Qiagen QIAgility, User Manual

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Appendix F
QIAgility User Manual 11/2011 F-5
Level sensor false trigger handling options
(aspirate)
False triggering occurs when the level sensor spuriously
reports that it has found the liquid surface when it hasn’t
actually reached the liquid during its downward probe. The
software considers all liquid surface levels found between the
tube base and the top of tube to be valid, and reports false
triggering errors when the level found by the sensor is above
the top of the tube. Such conditions can occur when static
electricity is present on the plasticware, as this may cause an
apparent change in capacitance similar to that caused when
the tip makes contact with liquid. False triggering which
occurs within the tube is undetectable.
False triggering of the level sensor which occurs during
aspirate operations usually causes insufficient volume to be
aspirated and hence a deficiency in volume transferred to the
target well.
This function presents options to reduce the impact of false
triggering caused by static electricity. Recommended settings
cause the pipettor to probe the surface at least twice looking
for successive level detections which are within a small
tolerance before proceeding with the aspirate.
By default, recommended settings to multiprobe the surface
are activated.
Level sensor false trigger handling options
(eject)
False triggering of the level sensor which occurs during eject
operations may cause insufficient volume to be delivered to
the target well.
This function presents options to reduce the impact of false
triggering caused by static electricity. Recommended settings
cause the pipettor to probe the surface at least twice looking
for successive level detections which are within a small
tolerance before proceeding with dispensing.
By default, recommended settings to multiprobe the surface
are activated.