Qiagen QIAgility, User Manual

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QIAgility Software
QIAgility User Manual 11/2011 5-145
5.10.5 Checking the reaction setup
A reaction setup can be checked in 2 ways:
1. Run a simulation of the reaction setup in “Virtual Mode”.
See Sections 5.1 and 5.2 for more information about
“Virtual Mode” operation.
2. Place the cursor over a source well to check the
destination well(s) of the source well. The destination
wells will be highlighted. For instance, if the cursor is
placed over a master mix well, the wells on the reaction
plate that will contain that master mix are highlighted.
5.10.6 Required volumes for reaction setup
The software automatically calculates the volume of
standard, diluent, reagents, and master mix required for a
reaction setup. To view the volume of liquid required,
generate the pre-run report. This report will list the required
volumes. See Section 6.7.1 for more information about the
pre-run report.
Alternatively, position the cursor above a source well (i.e.,
diluent, reagent, master mix, or standard). A hover box will
appear that reports the volume required. This volume
includes an extra volume, to account for the dead volume,
that is dependent on the tube type.
5.11 Selecting the COM port
1. Select “Options/Robot Setup/Select COM port”.
2. The “Configure Serial Interface” dialog box will open.