Qiagen QIAgility, User Manual

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QIAgility Software
QIAgility User Manual 11/2011 5-89
Preserve source
well indices?
This function is visible only when enabled
in the “Advanced Settings” menu in the
“Options” menu. When selected, the
reaction constructed for each sample is
placed into the same relative location
from the start of the reaction plate as the
sample from the start of the sample plate,
determined according to pipetting order.
Mix on
ejection?
Instructs the instrument to perform a mix
cycle upon ejecting the sample and
defines the number of mix cycles to
perform. Mixing is only performed when
the final reaction component is added,
and only when there is more than 1
component in the reaction.
Use
normalization?
Instructs the instrument to normalize
sample concentrations.
Auto Adjust
small Volumes
Instructs the instrument to round up
sample volumes that are less than the
minimum volume that can be pipetted
(i.e., 1 µl), to the minimum volume (i.e.,
1 µl). Where the operation would require
less than the pipettable volume of diluent,
no diluent is transferred, only sample is
moved. In all such cases, the final volume
is preserved, but the desired final
concentration is not achieved.
Normalize to
conc.
Defines the concentration to which
samples will be normalized. This excludes
the contribution that other reagents and
master mix make to the reaction.
Setting up a reaction list
Note : If the total number of reactions configured on one
plate exceeds the number of reaction wells a warning will