Qiagen QIAgility, User Manual

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Appendix E
E-2 QIAgility User Manual 11/2011
detector/reporter/quencher sets. To achieve this, master
mixes used in the exported reactions must be named in the
QIAgility software according to the convention:
M:D_R_Q:D_R_Q
“M” is the marker name. All other items are the same as for
“Absolute Quantification”.
For example, “Mix D:CMV_FAM_TAMRA:EBV_JOE_TAMRA”
specifies a mix with detector for CMV using FAM reporter
and TAMRA quencher, and detector for EBV using JOE
reporter and TAMRA quencher. “Mix D” represents the
marker name in the SDS software.
The allelic discrimination function exports 2 files, “XXX.TXT”
and “XXXend.TXT” (for endpoint analysis), where “XXX” is the
filename.
Note : The “Absolute Quantification” and “Allelic
Discrimination” export modes ignore reactions where the
master mix name does not match the required format. In the
resultant files, wells are only listed where their master mix
contents are named, so that they can match the required
format.