Qiagen QIAgility, User Manual

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QIAgility Software
QIAgility User Manual 11/2011 5-67
also be defined. The software calculates the volume of
reagent and diluent required to make the correct volume of
master mix.
If premade master mix is used, the user only needs to define
the volume of master mix per reaction.
Note: Preset master mixes can only be loaded if they consist
of maximal 16 ingredients.
Note : Commercially available master mixes are not
premade master mixes because they require addition of
primers. A commercial master mix, and the individual
primers, must be defined as reagents in the software and
then combined into a master mix by the QIAgility.
By default, master mixes are located on the Master Mix
Block, but they can also be located on other plates. Master
mixes are set up using the “Mix” right-hand pane. To view
the “Mix” right-hand pane, left-click on any well with “Mix”
function.
“Mix” right-hand pane