LC Packings Probot 161403, Руководство пользователя

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Installation and Getting Started
User’s Manual Probot
D960R3
2-9
2.4.2 Fluidic Connections for Fraction Collection
2.4.2 A Connecting the µ-Tee for Matrix Addition
The Probot Micro Fraction Collector should be installed as near as possible to the
UltiMate Capillary HPLC system. Since the µ-Tee is located in the center of the
cover of the unit, the Probot can be placed on either side of the LC system.
The µ-Tee as originally installed in the Probot is equipped with 30 µm I.D.
(280 µm O.D.) fused silica fractionation needle which allows for a maximum flow
rate of up to 30 µL/min (matrix solution and LC flow). To reconfigure the
instrument for a maximum flow rate up to 300 µL/min, refer to Section 3.3.5.
CAUTION
Caution: Exceeding the maximum flow rate limits specified in Section 3.3.5 may
cause the rotary valve to leak or may damage the rotary valve.
To connect the Probot µ-Tee to the flow cell outlet of the UltiMat e UV Detector:
a) Depending on your application (e.g. capillary or Nano LC) use either a 30
µ
m
I.D. (280 µm O.D.) fused silica capillary (P/N 161473, for Nano LC) or a 100
µ
m I.D. (280 µm O.D.) capillary (P/N 160479, for capillary LC) to extend the
outlet capillary of the flow cell (not included in the accessory kit).
b) Install the fused silica capillary into the upper port of the µ-Tee (FIGURE 2-4)
inside the Probot using one long orange PEEK sleeve (P/N 162146), which is
provided with the Probot. Turn the fitting until it is finger tight. You should
not be able to move the capillary in the sleeve (e.g. it should be grabbed by
the sleeve).
Fused Silica Capillary
PEEK Sleeve (long)
X
Ferrule
µ-Tee
Fused Silica
Needle
Stainless Steel
Needle
Inlet for Matrix
Solution
X = 2 - 3 mm
I.D. of the
Fractionation Needle
Maximum Flow Rate
( LC flow)
30 µm 30 µL/min
100 µm 300 µL/min
PEEK Sleeve (short)
FIGURE 2-4 The µ-Tee
c) Use a Microtight ® union (P/N 161497, not included in the accessory kit) and
two orange PEEK sleeves (P/N 161498) to connect the capillary to the outlet
of the flow cell (280
µ m O.D.).