LC Packings Probot 161403, Руководство пользователя

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Maintenance and Troubleshooting
User’s Manual Probot
D960R3
3-3
3.3 Replacing Major Components
A variety of components on the Probot Micro Fraction Collector can be readily
changed by the user as required to ensure that the instrument is maintained in
optimal condition. In some circumstances the analyst may want to alter the
configuration of the system (e.g. installing the pipetting option, Section 3.3.1).
In most cases, re-assembly of a component is identical to its disassembly, except
that the steps are performed in the reverse order. If no comment is made, it
should be assumed that the assembly of a component or installation of a
component is identical to disassembly or removal, except that the actions are in
the reverse order.
WARNING
Warning: Hazardous voltages are present inside the instrument. Disconnect the
instrument from the electrical supplies before removing any panel or cover.
3.3.1 Installing the Pipetting Option
The pipetting option (P/N 161072) allows for pipetting from a source plate (e.g.
a well plate) to a destination plate (e.g. MALDI target). It consists of a Teflon
tube (45 cm, I.D. 250
µ m) with fittings and a fused silica capillary (15 cm, O.D.
280 µ m, I.D. 100 µ m).
max.
4 mm
Flat Ferrule
Fused Silica Capillary
Teflon Tubing
FIGURE 3-1 The Pipetting Option
a) Cut the fused silica capillary to the appropriate length.
b) Carefully insert the fused silica capillary all the way through the needle and
tighten the fingertight nut.
c) Connect the fingertight fitting with the flat ferrule to port 1 (PUMP) of the
rotary valve.
Note: For pipetting volumes less than 500 nL, the fused silica should be as short
as possible. In such a case, cut it to the minimum length required and insert the
fused silica capillary. The part of the capillary in the Teflon tube should be less
than 4 mm long.