LC Packings Probot 161403, Руководство пользователя

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Installation and Getting Started
2-24
D960R3
User’s Manual Probot
the time elapsed after the task was started exceeds this limit. In the case all
spots have been processed, the run also stops. In both cases µCarrier will
prepare the next task. The Time per spot(s) is the actual spotting time. This
time includes the time the Probot needs to move, to lift the table and to spot
(t
spotting
= t
movement
+ t
on spot
). It can be as low as 4 seconds (with optimized
needle movement only).
•
3 – Where… - The positions (groups) are defined via this box. The Collect
group is where the Probot collects the LC eluent (with the matrix solution).
The Standby position is where the needle rests before and after the spotting.
The Z-coordinate for in-task movement defines the height at which the table
moves from spot to spot (‘0’ represents the lowest position of the table). A
typical value is approximately 2 mm below the spotting height (which was
defined during the sample carrier alignment step, Section 2.7.3).
CAUTION
Caution: The Z-coordinate for in-task movements must ALWAYS be lower than
the spotting height (Section 2.7.3). If it’s not lower, you may scratch your
MALDI target.
Note: For faster spotting (e.g. the spotting time needs to be decreased), the
Z-coordinate for in-task movements should be as close as possible to the spotting
height (Section 2.7.3).
Check all tasks in the task list and save the application under any appropriate
name.
µ Carrier is now ready to fractionate. To run the application in this stage,
continue with step c) of the instructions provided in Section 2.7.5.
2.7.5 Running a predefined Application
To load and to run a predefined application, perform the following steps:
a) Double-click on the application you want to run in the browser window
(FIGURE 2-13) or use the Open file button in the main menu toolbar.
b) The Application Editor and the Tray Editor is updated accordingly.
CAUTION
Caution: To verify your application, we recommend that you remove the needle
and all parts from the table so that they cannot be damaged during the
verification process.
c) Start the application by selecting the Run the application button. You can
pause the application by choosing the Pause the application button or you
can stop the application by choosing the Abort the application button.