LC Packings Probot 161403, Руководство пользователя

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Appendix B
B-2
D960R3
User’s Manual Probot
B.2 Contact Closure Signals for Peak Detection with
CHROMELEON
The following example uses three relay outputs of the UltiMate Micropump. The
relay 1 (event output 7) will generate the ‘Peak Start’ signal while relay 2 (event
output 8) will generate the ‘Peak End’ signal. The OC1 output (event output 3) is
used to generate the ‘START’ signal for the remote start of the
µ
Carrier
software. The pulse length of all signals is 1 second.
The peak start will be generated if the slope of the UV signal exceeds a certain
limit (e.g. 0.1 mAU/sec), the peak end signal will be generated if the UV signal is
lower than a certain limit (e.g. 1.0 mAU) and if the slope of the UV signal drops
beyond a certain limit (e.g. - 0.03 mAU/sec).
The following listing shows the programming of these limits, a complete listing of
this example is presented in Section B.5.
…
Trigger peakstart UV_VIS_1.Delta > 0.1, true=2.00
Pump_Relay_1.State = On
Pump_Relay_1.Duration = 1.00
EndTrigger
Trigger peakend UV_VIS_1 < 1.0 and UV_VIS_1.Delta > -0.03, true=2.00
Pump_Relay_2.State = On
Pump_Relay_2.Duration = 1.00
EndTrigger
…
The parameters “UV_VIS_1.Delta” and “UV_VIS_1” can be used independently,
you can define either or both and they need to be modified according the
requirements of your application. FIGURE B-1 shows a chromatogram obtained
with the method presented in Section B.5. The triggers are shown as vertical
lines (using the appropriate CHROMELEON options in the ‘decoration’ menu).
0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0 22.0 25.0
-1.00
-0.50
-0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
PEPMIX TFA CON TUB 30 EN 50 UM for Probot #10 pepmix 1 ng/ul UV_VIS_1
mAU
min
1 - 10.183
2 - 10.892
3 - 11.883
4 - 11.975
5 - 13.250
6 - 14.067
7 - 16.750
8 - 17.167
WVL:214 nm
peak
s
tar
t
peak
end
peak
s
tar
t
peak
end
peak
s
tar
t
peak
end
peak
s
tar
t
peak
end
peak
s
tar
t
peak
end
peak
end
peak
s
tar
t
peak
end
peak
s
tar
t
peak
end
peak
s
tar
t
peak
end
FIGURE B-1 Chromatogram with Triggers indicated