Zeiss TIRF 3, Operating Manual

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Carl Zeiss Contents Laser TIRF 3
4 423683-7144-001 02/2014
3.4 Mounting the Laser Safety Cover for Reflected Light Provided for Mechanical and
Scanning Stages ................................................................................................................57
3.5 Mounting the S TIRF S1/S TIRF DARK S1 Incubator .............................................................58
3.6 Incubator XLmulti S1 RED LS and Incubator XLmulti S1 DARK LS.........................................60
3.6.1 Laser Safety: Position of the Reed Contacts ........................................................................ 62
3.6.2 Mounting ......................................................................................................................... 62
3.7 PM S1 LS Incubators ..........................................................................................................65
3.7.1 Mounting ......................................................................................................................... 66
3.7.2 Assembly of the Magnet Screw ......................................................................................... 68
3.8 S TIRF S1 and S TIRF DARK S1 Incubators ...........................................................................69
3.9 Insert Slider Laser TIRF .......................................................................................................70
4 OPERATION ......................................................................................................... 71
4.1 Control and Functional Elements .......................................................................................71
4.1.1 Manual Slider Laser TIRF / mot. .......................................................................................... 71
4.1.2 Laser Bench Tower ............................................................................................................ 72
4.1.3 Laser Bench Rack .............................................................................................................. 73
4.2 Switching on .....................................................................................................................74
4.3 Switching off ....................................................................................................................75
4.4 ZEN Software (blue edition) ...............................................................................................75
4.5 Checking and Adjusting the Laser Beam Divergence and the TIRF Angle .............................75
4.5.1 Delivery Scope of the TIRF Adjustment Tool Kit ................................................................... 75
4.5.2 Adjusting the Laser Beam Divergence Using the Adjustment Tool ....................................... 77
4.5.3 Adjusting and Checking the TIRF Angle Using the Adjustment Tool .................................... 78
4.6 Adjusting the TIRF Effect on the TIRF Slider man. ................................................................79
4.7 Controlling the Laser TIRF Imaging System Using ZEN 2012 (Blue Edition) ...........................81
4.8 Calibration of the Angle Using the Motorized Slider Laser TIRF ...........................................82
4.8.1 Measurement of Penetration Depth ................................................................................... 82
4.8.2 Snell's Law ........................................................................................................................ 82
4.8.3 The Angle-Voltage Relationship of the Slider Laser TIRF ...................................................... 83
4.8.4 Determining the Critical Angle ........................................................................................... 84
4.8.5 Determination of the Refractive Index of the Specimen ....................................................... 84
5 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE .............................. 85
5.1 Instrument Care ................................................................................................................85
5.2 Instrument Maintenance ...................................................................................................85
5.3 Troubleshooting ................................................................................................................86
5.4 Fuse Replacement .............................................................................................................87
5.4.1 Signal Distribution Box ...................................................................................................... 87
5.4.2 Laser Bench Tower ............................................................................................................ 87
5.4.3 Laser Bench Tower coupling module .................................................................................. 88
5.5 Changing the HAL Halogen Lamp ......................................................................................88
5.6 Retrofitting the Laser Units ................................................................................................88