Zeiss TIRF 3, Operating Manual

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Laser TIRF 3 Contents Carl Zeiss
02/2014 423683-7144-001 3
CONTENTS
Page
1 DEVICE SAFETY ...................................................................................................... 6
1.1 General Notes .................................................................................................................... 6
1.2 Safety Instructions .............................................................................................................. 8
1.2.1 Laser Radiation ................................................................................................................... 8
1.2.2 Installation Instructions ....................................................................................................... 9
1.2.3 Notes on Computer and Software ..................................................................................... 11
1.2.4 Service and Customer Interfaces ....................................................................................... 12
1.2.5 Warning and Information Labels on the Instrument ........................................................... 18
1.3 Notes on Warranty ........................................................................................................... 22
2 INSTRUMENT DESCRIPTION ................................................................................ 23
2.1 Intended Use .................................................................................................................... 23
2.2 Operating Principle ........................................................................................................... 24
2.3 Instrument Description and Main Features ......................................................................... 26
2.3.1 Slider Laser TIRF, man. / mot. ............................................................................................ 26
2.3.2 Laser Bench Tower and Laser Bench Rack or Laser Bench Rack 3F ...................................... 28
2.3.3 Signal Distribution Box ...................................................................................................... 29
2.3.4 TIRF Controller ................................................................................................................. 29
2.4 Laser Safety for the Sample Compartment ......................................................................... 30
2.5 Basic kit laser safety Rev. 2 ................................................................................................ 31
2.6 Remote switch for laser mode ........................................................................................... 32
2.7 Laser Safety Concept ........................................................................................................ 33
2.8 Configuration Overview of the Laser TIRF Imaging System ................................................. 34
2.9 System Overview Laser TIRF Imaging System ...................................................................... 35
2.9.1 Compatibility of Stages and Laser Safety Devices ............................................................... 39
2.9.2 Compatibility of XLmulti Incubators and Accessories .......................................................... 40
2.9.3 Overview of Stage Versions for TIRF in Axio Observer Program ........................................... 41
2.10 Objectives to Be Used with the Laser TIRF Imaging System ................................................. 42
2.11 Fluorescence Filter Sets to Be Used with the Laser TIRF Imaging System .............................. 43
2.12 Combination of Laser TIRF Imaging System and Colibri.2 Illumination System ..................... 46
2.13 Optical Fibers to Be Used with the Laser TIRF Imaging System ............................................ 46
2.14 Technical Data .................................................................................................................. 47
3 START-UP ............................................................................................................. 52
3.1 Installation Requirements .................................................................................................. 52
3.1.1 Space Requirements ......................................................................................................... 52
3.1.2 Power Requirements ......................................................................................................... 53
3.1.3 Ambient Conditions ......................................................................................................... 55
3.2 Requirements for the Microscope Stand ............................................................................ 55
3.3 Basic Microscope Settings ................................................................................................. 56