INSTRUMENT DESCRIPTION
Carl Zeiss
Objectives to Be Used with the Laser TIRF Imaging System
Laser TIRF 3
42
423683-7144-001
02/2014
2.10
Objectives to Be Used with the Laser TIRF Imaging System
The functional principle described in Section 2.2 makes specific demands on the correction state of the
objectives used.
From the relation between the angular aperture of an objective and the numerical aperture (NA):
)
(sin
*
φ
n
NA
=
Φ
half the angular aperture
n
refractive index 1
it follows that only an objective with a high numerical aperture of 1.33 (corresponds to 61°) achieves the
critical angle of total reflection at a glass/water boundary layer.
As the laser beam itself also has an aperture (divergence), a numerical aperture of the objective larger
than 1.33 must be selected to ensure that sufficiently high laser power will be emitted to the specimen.
For this reason, the method is optimally suited for combinations with the following objectives:
Thread size
Contrast
Objective lens
M27
W0.8
TIRF
H
Ph
DIC
ACR
Objective
α
Plan-Apochromat
63x/1.46 Oil Korr M27
(420780-9970-000)
+
–
+
+
–
DIC slider EC PN
63x1.25 III,
CA 63x/1.2 W III
(426946-0000-000)
424510
Objective
α
Plan-Fluar 100x/
1.45 Oil M27
(421190-9900-000)
+
–
+
+
–
DIC slider
α
Plan-Fluar
100x/1.45 III
(000000-1131-718)
DIC slider Fluar
100x/1.45 HC II
(426953-0000-000)
424509
Objective
α
Plan-Apochromat
100x/ 1.46 Oil DIC M27
(420792-9800-000)
+
–
+
+
–
DIC slider PA
100x/1.46 III
(426963-0000-000)
424509
Objective
α
Plan-Apochromat
100x/1.46 Oil DIC M27;
with segment diaphragm
(420792-9800-720)
+
–
+
+
–
424509
Objective
α
Plan-Apochromat
100x/1.46 Oil Ph3 M27
(420791-9810-000)
+
–
+
+
+
424509
Objective
α
Plan-Apochromat
100x/1.46 Oil Iris M27
(420796-9800-000)
+
–
+
+
–
424510
Objective
α
Plan-Apochromat
100x/1.46 Oil DIC
(440782-9800-000)
–
+
+
+
–
–
Objective
α
Plan-Apochromat
100x/1.57 Hi-Oil DIC Korr
M27,with segment diaphragm
(420792-9771-000)
+
–
+
+
–
DIC slider PA
100x/1.57 III
(426967-9080-000)
424510
+ = Relevant feature i.e. combination possible
−
= Non-relevant feature i.e. combination not possible
Summary of Contents for TIRF 3
Page 1: ...Laser TIRF 3 Imaging System Operating Manual...
Page 110: ......
