Zeiss TIRF 3, Operating Manual

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INSTRUMENT DESCRIPTION
Laser TIRF 3 Suitability of Fluorescence Filter Sets for the ... Carl Zeiss
02/2014 423683-7144-001 43
2.11 Fluorescence Filter Sets to Be Used with the Laser TIRF Imaging System
The following fluorescence filter sets are suitable for TIRF microscopy with Laser Bench Tower or Rack and
in combination with SIRF (HBO-TIRF):
Wavelength
(nm)
Laser
TIRF
SIRF
Filter set
No.
Type Designation Order No.
Typical
dyes
365 - ++ 49 SBP
Filter set 49 DAPI shift free
EX G 365, BS FT 395,
EM BP 445/50
488049-9901-000 DAPI
405
++ + 87HE SBP
Filter set 87 HE YFP shift free
EX BP 406/32, BS FT 427,
EM BP 459/25
489087-0000-000
- ++ 70HE SBP
Filter set 70 HE Alexa 430 shift
free (E)
EX BP 430/60, BS FT 500, EM BP
550/100
489070-0000-000 CFP
++ O* 72HE TBP
Filter set 72 HE CFP/YFP/DsRed
shift free
EX TBP 407+510+565;
BS TFT 431+525+580;
EM TBP 468+537+604
489072-0000-000
CFP,
YFP,
mRFP,
dsRED
++ O* 73HE DBP
Filter set 73 HE CFP/YFP shift free
EX DBP 410/39+509/18;
BS DFT 443+523;
EM DBP 470/20+554/50
489073-0000-000 CFP/YFP
++ O* 76HE TBP
Filter set 76 HE CFP/YFP/DsRed
shift free
EX TBP 406+489+561;
BS TFT 427+503+578;
EM TBP 459+525+608
489076-9901-000
CFP/GFP,
DsRed
445
++ + 85HE SBP
Filter set 85 HE CFP shift free
EX BP 445/25, BS FT 463,
EM BP 506/75
489085-0000-000
++ - 84HE TBP
Filter set 84 HE
(445+515+638nm) shift free (E)
EX TBP 434/40+517/18+640/22,
BS TFT 460+532+659,
EM TBP 482/34+576/78+688/45
489084-0000-000
458*
++ + 51 SBP
Filter set 51 (458nm) shift free
EX BP 458/10, BS FT 470,
EM BP 485/30
488051-0000-000
Cy2
FITC
Alexa 430
CFP
ECFP
+ ++ 37 SBP
37 Wild Type GFP shift free
EX BP 450/50, BS FT 480,
EM BP 510/50
000000-1031-342
O + 44 SBP
44 FITC special, d=25 shift free
EX BP 475/40, BS FT 500,
EM BP 530/50
000000-1114-459
- + 47HE SBP
Filter set 47 HE CFP shift free
EX BP 436/25, BS FT 455,
EM BP 480/40
489047-9901-000
* = Wavelength not available for Laser Bench Tower or Rack