OPERATION
Laser TIRF 3
Calibration of the Angle Using the Motorized Slider Laser TIRF
Carl Zeiss
02/2014
423683-7144-001
83
4.8.3
The Angle-Voltage Relationship of the Slider Laser TIRF
The following correlation applies for the deflection of the laser beam in the rear objective pupil:
1
0
64
.
1
015
.
2
)
sin(
xn
x
U
U
−
=
α
The numerical term of the denominator consists of microscopic characteristics and the imaging ratio of
the laser.
The angle is adjusted by means of a piezo actuator in the Slider Laser TIRF. The actuator is operated with
analogue potential from -10 to +10 Volts. At 0 Volt the laser beam should ideally appear at 0° above the
sample - i.e. vertically above it. Every angle in the sample therefore corresponds to a voltage.
Tolerances in the beam path, e.g. the position of the reflector module and the position of the specimen,
can result in an offset voltage (U
0
) having to be created to ensure that the laser beam appears directly at
0° above the specimen. This offset is determined and saved in a calibration wizard in the imaging
software´ for each objective and the corresponding reflector modules and filter sets used. The correct
angle is then available in the software.
How does calibration work?
mean
Specimen plane
Immersion oil
Rear focal plane
Optical axis
Summary of Contents for TIRF 3
Page 1: ...Laser TIRF 3 Imaging System Operating Manual...
Page 110: ......
