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OPERATION
LSM 510
Image Acquisition (Acquire)
B 40-051 e 07/98
4-35
4.5.2.2
Epi-fluorescence observation (Axioplan 2
MOT
)
Turn on the HBO 100 W power supply with switch (4-37/2).
Push in the silver slider (4-37/8) on the microscope tube as far as it will go.
This opens the light path for specimen observation through the eyepieces.
Switch on the reflected light.
Pull out the occluding slider (4-37/1) to a light-passing position; actuate shutter switch (4-37/4) for
reflected light if it is in transmitted-light position.
☞
To avoid excessive bleaching, expose the specimen to the minimum possible irradiation, i.e.
keep the irradiation time as short as possible.
In the
Reflector Turret
list box, select the reflector module (filter sets) to suit the type of fluorescence
excitation. Proceed as follows:
Open the
Reflector Turret
list box.
Click on the desired reflector module.
The reflector turret moves the selected
reflector module into the beam path.
☞
The FITC filter set (FSET09) consists
of an excitation filter for the
450 - 490 nm spectral range, an
FT color splitter for 510 nm and an
LP long pass filter, which passes
emission light wavelengths greater
than 510 nm.
Fig. 4-38
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...