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INTRODUCTION TO LASER SCANNING MICROSCOPY
Optical Diagram of the LSM 510
LSM 510
3-6
B 40-051 e 07/98
3.3
Optical Diagram of the LSM 510 (Schematic)
Fig. 3-2
Optical path, schematic (4-channel configuration)
AOTF Acousto Optical Tunable Filter
DBC
Dichroic Beam Combiner
DBS
Dichroic Beam Splitter
EF
Emission Filter
HAL
Halogen Lamp
HBO
Mercury Vapor Short-Arc Lamp
LSF
Line Selection Filter
MDBS Main Dichroic Beam Splitter
NDF
Neutral Density Filter
VP
Variable Pinhole
PMT
Photomultiplier
T-PMT Transmission-Photomultiplier
The diagram above is a schematic representation of the LSM system.
Laser light is focused onto the specimen through an objective in a diffraction-limited mode. Light emitted
at the focal plane and at planes below and above it is directed via an XY scanner onto a main dichroic
beam splitter (MDBS), which separates the emissions from the excitation light. The fluorescences are
separated from each other by a series of dichroic beam splitters (DBS1 ... maximally DBS4) and directed
to individual photomultipliers (PMT1 ... maximally PMT4).
Fiber Coupler
AOTF
Shutter
Eyepiece
Tube Lens
Tube Lens
HBO
Objective
Specimen
Condensor
T-PMT
HAL
Scan
Lens
Scanner
y
X
Mirror
DBC
Plate
Collimator
LSF NDF
Monitor
Diode
Pinhole
Optics
MDBS
DBS2
DBS1
DBS3
PMT3
PMT4
VP4
EF4
VP2
EF2
VP1
EF1
VP3
EF3
PMT2
PMT1
Ar-UV Laser
Ar/ArKr Laser
HeNe Laser
HeNe Laser
UV Fiber
VIS Fiber
Fiber Coupler
AOTF
Shutter
Laser
Module VIS
Scan Module
Microscope
Laser
Module UV
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...