OPERATION
Quick start
LSM 510
4-18
B 40-051 e 07/98
(17) To further improve image quality you can slow the scan rate allowing more photons to integrate
on the detector, or apply image averaging to remove random noise, or a combination of both.
These adjustments are made by clicking on the <Mode> button on the
Scan Control
menu. Set
the
Scan Speed
in the
Speed
area and
Number
in the
Depth, Scan Direction & Scan Average
area accordingly by observing your image. The setting average of 8 (Number 8) should improve
signal/noise dramatically, however, the image acquisition rate will be slower.
(18) If your specimen is sensitive to photobleaching, you can attenuate the laser illumination by clicking
on the <Channels> button from the
Scan Control
menu. At the bottom of the menu you can set
the percentage of laser power (
Excitation %
) for each excitation wavelength. You will probably
have to inrease the
Detector Gain
if you decrease the laser power. This setting controls the
transmission degree of the AOTF.
(19) To save your image click on the <Save> or <Save As> button on the right side of the image.
Fig. 4-18
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...
