OPERATION
LSM 510
Image Acquisition (Acquire)
B 40-051 e 07/98
4-49
Laser attenuation
On the
Beam Path and Channel Assignment
panel, move the cursor to the <Excitation>
button.
Once the cursor has changed into a hand
symbol, click on the button.
This opens a drop-down list of all available
lasers with their wavelengths and their
usable AOTF attenuation.
To select the desired laser, click on the
respective line in the list.
The selected laser will be highlighted in blue.
Use the Excitation [%] slider to set the laser
power (recommendation: start at 50 %).
The transmittance of the Acousto-Optical
Tunable Filter (AOTF) changes accordingly.
☞
If you deactivate "Line On", the laser wavelengths for Enterprise and argon lasers are
deselected by means of the AOTF, i.e. these lasers change into standby status.
If you interrupt your work with the LSM for a break, it is recommended not to switch the
Enterprise and argon lasers off by hardware action, but to put them into standby status as
described.
Scanning operation is not possible in the standby status.
Operate the Excitation slider as follows:
Click on the slider and shift it while keeping the left mouse button pressed.
Keep the <Shift> key pressed and click on the
or
arrow button to vary the power in steps of
ten.
Keep the <Caps Lock> key pressed and click on the
or
arrow button to vary the power in steps
of one.
Keep the <Ctrl> key pressed and click on the
or
arrow button to vary the power in steps of one
tenth.
Fig. 4-49
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...