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INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510
Overview and Explanations
B 40-051 e 07/98
5-3
5
3D FOR LSM 510
5.1
Overview and Explanations
5.1.1
The Image Sequence
The "3D for LSM" handles image sequences
generated by the Zeiss LSM software. This can be
three-dimensional image data or a time sequence
of two-dimensional images (slices). Each slice (as
well as the sequence) can consist of up to eight
channels. An image sequence consists of a series
of individual (2D) images and has a name that
designates the entire sequence. In general an
image sequence is handled as a single object in the
system. Individual channels or slices can be
addressed.
The following terms and definitions apply for the
"3D for LSM" software.
An image sequence is a number of individual
sequential images (usually called slices in the
dialog boxes), the spacing between which is
equal.
Image sequences can contain up to 12 bit of
image data (per channel).
A sequence (slice) can consist of up to eight
channels.
The maximum size of an image sequence is
limited by the provided memory of the operating system.
A voxel is the smallest element of an image sequence (the equivalent of a pixel in a 2D image). All
voxels in a given image sequence are the same size.
The coordinate system originates in the left upper front corner of the image sequence. This point has
the coordinates 0, 0, 0.
All angles are positive for rotations to the right in the direction of the positive coordinate axis (right-
handed coordinate system).
A slice is an individual image in a sequence of images. The numbering of the slices starts with "1".
Single slice with
single channel
Image sequence
Multichannel
(0, 0, 0)
Single slice with
multiple channels
Voxel
Positive rotation directions
of the axes
Fig. 5-1
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...