OPERATION
Image Optimization
LSM 510
4-100
B 40-051 e 07/98
For overlaying fluorescence and transmitted-light
images, click on the <Transmission> button on the
Beam Path and Channel Assignment
panel.
The transmitted light PMT will be switched active.
Of course, all other transmitted light applications
like
phase contrast
differential interference contrast (DIC)
polarization contrast (Pol)
darkfield
can also be performed.
Image optimization must be performed as
described in the previous chapter.
For the generation of images in reflection, the main dichroic beam splitter must be a neutral-density
filter.
Standard equipment contains a neutral-density filter with a division ratio of 80 to 20 % (at 543 nm).
Fig. 4-108
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...