OPERATION
LSM 510
Image Acquisition (Acquire)
B 40-051 e 07/98
4-57
4.5.4.2 Z
mode
This mode allows you to create a stack of images
at different Z levels (slices).
In the "Scan Control" window on the
<Z Stack> button.
This opens the
Z Settings
panel
in the
"Scan Control" window.
The scanning range in Z can be determined as an
option via the following functions:
Z Sectioning (input of numerical values)
Cutline and Range (in monitor dialog with
the specimen)
Mark First/Last (in monitor dialog with the
specimen)
Z Sectioning
Activate the Z Sectioning register
Use the
Num Slices
slider to select the number
of slices in the stack.
Use the
Interval [µm]
slider to select the
Z interval between successive slices.
Use the
Current Slice
slider to select the center
of the stack.
Click on the <Start> button to trigger Z Sectioning.
Optimization of intervals, number of slices and
pinhole diameter
Click on the <Z Slice> button to determine the
optimum interval
The
Optical Slice
dialog box (see Fig 4-59) will
appear, from which the optimum interval
(depending on the used objective and pinhole
diameter) can be seen and made available to the
active mode.
Clicking on the <Optimal
Pinhole
Diameter>
button also enables the optimum pinhole diameter
to be set.
Fig. 4-57
Fig. 4-58
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...
