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INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510
Functions
B 40-051 e 07/98
5-47
Segment - Automatic
The function carries out an automatic grey value segmentation by means of thresholding.
Fig. 5-25
The
Automatic
tab sheet of the
Segment
dialog window must be selected. Segmentation is especially
used to generate binary regions. These are required for the measurement.
The function calculates the two strongest local minimums in the histogram of the
Input
image
sequence. These values are used for the discrimination. Only one channel of a multichannel sequence
can be selected as
Input
.
Output
will always be a single channel sequence. The vertical scaling of the
histogram can be adjusted with the scroll bar at the right edge of the histogram. This setting has no
influence on the function.
The
Green
and
Blue/Red
option buttons of the parameter
Colour
determine whether the voxels within
(
Green
) or outside (
Blue/Red
) of the grey value interval [
L, H
] are displayed with the corresponding
colour.
If
Green
is selected, the voxels within the selected interval are highlighted in green. The rest of the
image retains its original grey values. The voxels with the grey values
L
ow and
L
ow+1 are displayed in
blue. The voxels with the grey values
H
igh and
H
igh-1 are displayed in red.
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...