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OPERATION
Image Acquisition (Acquire)
LSM 510
4-54
B 40-51 e 07/98
4.5.4 Scanning
modes
Taking a simple configuration as an example, the sections below describe the procedure for acquiring a
single scanned image in single-channel presentation, using fluorescence and confocal imaging.
Requirements
Axioplan 2
MOT
Push in the silver slider (4-37/
8
) on the microscope tube as far as it will go.
Activate the "Axioplan Control" window by mouse click, or, if the window was closed before, call it
up again by clicking on the <Micro> button.
Continue as described in sections 4.5.2.1 and 4.5.2.2.
The specimen must have been positioned and focused by conventional eyepiece observation in
fluorescent or transmitted light.
Axiovert 100 M BP
Perform the settings in the same way as described for the Axioplan. Make sure that the sideport slider
(4-39/
2
) and VIS slider (4-39/
3
) are in the correct positions.
Scanning
Click on the <Scan> button in the main menu.
This opens the "Scan Control" window, in
which all necessary scanning parameters can
be set.
The first selection to be made is whether you want
to perform a
Line scan
Frame scan
Use ROI or
Z stack.
This selection will be made via the <Mode>
button, followed by activation of the desired scan
mode.
The following description is limited to the Frame
and Z modes. For "Line Scan", proceed in the
same way.
Fig. 4-54
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...