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OPERATION
Annex
LSM 510
4-190
B 40-051 e 07/98
Examples of application:
a)
Checking the laser power
This function is not automized so far. To qualitatively measure the laser power the diode can be used in
such a way, that the graylevel obtained in the
Line Scan
mode at a certain setting of the whole system
is stored as a text overlay together with the image (manually done by user). As the diode setting (Ampl.
Gain, Ampl. Offset, ND filter) is stored together with the image, the setting is automatically reloaded
when using the <REUSE> button. If deviations can be observed it is easy to set the laser power to the old
value by means of the AOTF transmission.
b) Noise Reduction by Ratio
In contrary to the PMT signal, the signal of the monitor diode <is not modulated by any specimen
information. Thus it can be used to ratio the PMT signal to get rid of the laser noise (due to any laser as a
physical fact) and thereby improve the signal to noise ratio of the fluorescence or reflectance image. The
major condition which has to be fullfilled to use the monitor diode for this purpose, is, that the
dominating source of noise is laser noise. The signal of the monitor diode will always be dominated by
laser noise (independent of the power set at the laser, or the transmission set at the AOTF), whereas the
dominating source of noise in the PMT signal can also be the shot noise of light (shot noise especially
occurs in low light fluorescence application; as rule of thumb it can be noted, that the shot noise is
limiting the signal to noise ratio, if the PMT voltage has to be set to a value > 400V).
Note: Any kind of noise which can not be observed in both channels at a time will be amplified and not
reduced by the ratio process. Low or high frequency laser noise is the only source of noise which is
correlated in the PMT signal and the signal of the monitor diode.
Using the HeNe lasers, noise is extremly low, so that in all applications, if, the shot noise of light will
reduce the signal to noise will reduce the signal to noise ratio of the image. Low or high frequency laser
noise is mainly introduced, if the Ar, ArKr lasers are used at a tube current lower than 8A (Ar-Vis, ArKr)
or 20 A (Ar-UV) respectively.
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...