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OPERATION
LSM 510
Image Optimization
B 40-051 e 07/98
4-81
4.7 Image
optimization
Single frames
Described below is the example of the acquisition of an image, using an excitation wavelength of 488
nm and a fluorescence emission range above 505 nm.
Let the specimen be a thin section through a stem of Convallaria majalis (Lily-of-the-Valley). The
description applies to the use of the Axioplan microscope, and analogously also to the Axiovert.
Requirements
The suitable laser is switched on.
The specimen has been positioned and focused
for scanning.
The slide rod on the microscope tube has been
pulled out as far as it will go.
Click on the <Config> button in the
Acquire
main menu item.
This opens the "Configuration Control"
window.
Click on the <Recording Configuration> button. A
window with the same title will open where you
can select the relevant configuration and activate it
via the <Apply> key.
Open the list box.
Select the necessary configuration (e.g.
488 nm, LP 505).
Activate the selected configuration by clicking
on the <Apply> button.
The
Beam Path and Channel Assignment
panel
displays the configuration loaded.
☞
The set laser intensity can be subsequently optimized for the current situation via the
<Excitation> button.
Fig. 4-90
Summary of Contents for LSM 510 Inverted
Page 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Page 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Page 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Page 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Page 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Page 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Page 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Page 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Page 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Page 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...