ZEISS
6 Operation | 6.2 Obtaining a First Image
6.2.2.4 Mounting the Specimen Holder
Safety Information
WARNING
Suffocation hazard: Lack of oxygen
Gaseous dry nitrogen is used to vent the specimen chamber during specimen exchange. Inhal-
ing nitrogen may cause unconsciousness.
4
During specimen exchange, keep the chamber door open as short as possible.
4
Do not inhale the air from within the specimen chamber.
4
Ensure that the area around the microscope is sufficiently vented.
4
If you begin to experience symptoms of asphyxia (for example: rapid breathing, loss of
mental alertness and/or muscular coordination, depression of sensations, emotional insta-
bility, fatigue) leave the room immediately and inform the facility’s safety officer.
CAUTION
Risk of injury: Moving the specimen stage
Fingers can be trapped by the moving specimen stage.
4
Always close the chamber door before moving the specimen stage.
4
To remove parts fallen into or near to the stage use a tool (e.g. tweezers) instead of your
fingers.
CAUTION
Risk of injury: Closing the chamber door
Fingers can be pinched when closing the chamber door.
4
Use the door handle to close the chamber door.
4
Ensure not to get your fingers caught in the chamber door gap.
NOTICE
Risk of property damage: Short working distance
When opening the chamber door, the microscope or specimen can be damaged if the speci-
men stage is at a short working distance. If a BSD detector is inserted, it can be damaged as
well.
4
Always move the specimen stage to a long working distance before opening the chamber
door.
NOTICE
Risk of property damage: Contamination caused by fingerprints
Contamination caused by fingerprints can lead to vacuum deterioration or prolonged pumping
times.
4
Always wear lint-free gloves when touching the specimen, specimen holder, or stage.
1. Carefully open the chamber door.
2. If a specimen holder is mounted onto the specimen stage, remove it by sliding it out of the
dovetail rails.
3. Mount the prepared specimen holder by sliding it into the dovetail rails.
Make sure that the dovetail is placed in the correct orientation so that the flat side of the
dovetail of the specimen holder is flush with the milled edge of the specimen stage.
Instruction Manual ZEISS Crossbeam 550L, Crossbeam 550 | en-US | Rev. 3 | 349500-8122-000
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Summary of Contents for Crossbeam 550
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