Zeiss Crossbeam 550, Instruction Manual

Page: 101 / 136

ZEISS 8 Troubleshooting | 8.3 Chamber
8.3.4 Replacing the Chamber Door Seal
Purpose Possible reasons for replacing the chamber door seal are the following:
§
Chamber door does not close tightly
§
Bad chamber vacuum
Overview
The procedure contains the following steps:
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Venting the Specimen Chamber [
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70]
§
Replacing the O-ring [
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101]
§ Evacuating the Specimen Chamber [
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72]
8.3.4.1 Replacing the O-ring
WARNING
Suffocation hazard: Lack of oxygen
Gaseous dry nitrogen is used to vent the specimen chamber during specimen exchange. Inhal-
ing nitrogen may cause unconsciousness.
4
During specimen exchange, keep the chamber door open as short as possible.
4
Do not inhale the air from within the specimen chamber.
4
Ensure that the area around the microscope is sufficiently vented.
4
If you begin to experience symptoms of asphyxia (for example: rapid breathing, loss of
mental alertness and/or muscular coordination, depression of sensations, emotional insta-
bility, fatigue) leave the room immediately and inform the facility’s safety officer.
NOTICE
Risk of property damage: Contamination caused by fingerprints
Contamination caused by fingerprints can lead to vacuum deterioration or prolonged pumping
times.
4
Always wear lint-free gloves when touching the specimen, specimen holder, or stage.
1. Carefully open the chamber door.
2. On the inside of the chamber door, remove the chamber door O-ring.
NOTICE Risk of property damage: If you use a metal tool to remove the O-ring,
then you may damage the sealing surface. If necessary, then only use a plastic or
wooden tool to remove the O-ring.
3. Inspect the groove that holds the O-ring and remove any contamination.
4. Insert the new chamber door O-ring.
5. Close the chamber door.
8.3.5 Resetting the Touch Alarm
Purpose To prevent damage, a touch alarm is integrated in the microscope. If the specimen or the speci-
men holder touches the chamber walls, the detectors, or the objective lens, the stage is stopped
immediately. An audible warning sounds and an on-screen message is displayed.
Prerequisite
ü
The EM Server shows the message WARNING Stage Touching.
1. To accept the warning, click OK.
2. Move the stage in the reverse direction away from the touch.
Instruction Manual ZEISS Crossbeam 550L, Crossbeam 550 | en-US | Rev. 3 | 349500-8122-000 101