QIAcuity User Manual 06/2022
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g)
High copy number in NTC
Check the images or signal map for dust or other particles. In
case, wipe the plate with a lint-free tissue (optionally, use ethanol)
and re-image the plate.
h)
Lower RFU of negative partitions in
NTC/samples with low number of
positive partitions
The signal intensity might be lower in images with high number of
negative partitions. There’s no influence on the result analysis, as
the signal to noise is not affected.
i)
The confidence interval is wide
The number of valid partitions is low. Check the images for
blacked-out areas, that can occur, e.g., due to bad filling or
areas of low amplification (see section Image corrective
measures)
j)
Vertical stripes in the images
Please re-image the plate for proper image analysis
k)
Double positive or double negative
signals
The double positive or double negative signals could have
different root causes. One of the reasons for observing double
signal bands could be suboptimal assay designs, such as cross-
hybridization of probes to unspecific targets or secondary off-
target amplification products. Besides assays-related causes,
improper crosstalk compensation could also be the root cause.
An insufficient compensation or overcompensation of crosstalk
from neighboring channels could also result in extra signal
bands. To determine the main root cause, re-image the plate with
30% less exposure times for affected channel. If double bands
disappear or get much closer to each other after re-imaging, they
are most likely to be caused by improper crosstalk compensation
rather than assay-related issues.
l)
Fetch error while accessing the Users
list in User Management
If such error occurs, please contact QIAGEN Technical Service in
order to solve the issue.
Software
a)
The QIAcuity Software Suite does not
start
Check that the software is installed on the laptop. Check the
operating system. The QIAcuity Software Suite can only be
operated and Windows 10.
b)
Disc space is critical in the QIAcuity
Software Suite
Delete plates from the plates overview.
c)
User forgot password
Administrator to log-in, change the password for the user. If
administrator forgot the password, contact QIAGEN Technical
Service.
d)
Communication error between
QIAcuity instrument and software
This error occurs when the data received from the instrument does
not conform to the expected pattern.
Further investigations are required by a QIAGEN Field Service
Specialist to diagnose the problem with the instrument.
Please contact your distributor or QIAGEN Technical Service.
e)
Instrument software or Software Suite
is unresponsive
Re-start the QIAcuity instrument or the notebook where the
QIAcuity Software Suite is installed
f)
Startup of instruments displays an
error
The required plate recovery task cannot be performed because
there are no plate slots available in the tray. Remove all loaded
plates before you proceed. Press
Restart
to start recovery.
g)
Error 205 or Error 32
The error can occur in different situations:
A) Please make sure that the selected Plate type corresponds with
the entered barcode, if manually entered. If not matching, it will
lead to an error on the instrument (error 205).
B) Please make sure that after the first successful suite connection,
the instrument is restarted to allow automatic synchronization of
labware files.
h)
Error 490
The error can occur after a plate was processed and a failure in
image transfer to the Suite was detected. The Suite rejected a
data package due to improper format. Please see if all images
are available in the Suite. If you find images missing, add an
additional imaging step to recover the data.
Summary of Contents for QIAcuity 911000
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