Qiagen QIAcuity 911000, User Manual

Page: 197 / 314

QIAcuity User Manual 06/2022 197
Analyze section with 2 Replicate groups (A11, B11) and (A12, B12).
Replicates are analyzed as separate wells but in addition the mean concentration value and the CI
value of the mean concentration are provided on demand.
On the right side above the table there is a checkbox, which allows the user to show mean values
for replicates in addition. By default, the button is unchecked, and results are displayed without
mean values. When the button is checked the list still shows independent rows for each selected
well, but replicates are grouped together. Replicates from the same group are next to each other in
the list view. There are two additional columns indicating the mean values:
Mean concentration
Mean concentration for replicates is calculated as an average, i.e., the sum of concentrations
multiplied with well-specific cycled volume of selected wells divided by the multiplication count of
selected wells with the sum of cycled volumes.
𝐹𝐹𝐹𝐹𝐹𝐹𝐹𝐹𝐹𝐹𝐹𝐹𝐹𝐹 𝑓𝑓𝐹𝐹𝐹𝐹 𝐹𝐹𝑚𝑚𝐹𝐹𝑚𝑚 𝑐𝑐𝐹𝐹𝑚𝑚𝑐𝑐𝑚𝑚𝑚𝑚𝑐𝑐𝐹𝐹𝐹𝐹𝑐𝑐𝑐𝑐𝐹𝐹𝑚𝑚 ( 𝑐𝑐𝑐𝑐 𝑐𝑐𝑚𝑚𝐹𝐹 µ 𝐹𝐹 )) 𝐹𝐹𝑓𝑓 2 𝐹𝐹𝑚𝑚𝑐𝑐𝐹𝐹𝑐𝑐𝑐𝑐𝐹𝐹𝑐𝑐𝑚𝑚𝑟𝑟 =
�𝑐𝑐 (1) ∗ 𝑣𝑣 (1) + 𝑐𝑐 (2) ∗ 𝑣𝑣 (2) �
( 𝑣𝑣
(1) +
𝑣𝑣
(2))
𝐸𝐸𝐸𝐸𝐹𝐹𝐹𝐹𝑐𝑐𝐹𝐹𝑚𝑚 𝑓𝑓𝐹𝐹𝐹𝐹 𝐹𝐹𝑚𝑚𝐹𝐹𝑚𝑚 𝑐𝑐𝐹𝐹𝑚𝑚𝑐𝑐𝑚𝑚𝑚𝑚𝑐𝑐𝐹𝐹𝐹𝐹𝑐𝑐𝑐𝑐𝐹𝐹𝑚𝑚 ( 𝑐𝑐𝑐𝑐 𝑐𝑐𝑚𝑚𝐹𝐹 µ 𝐹𝐹
)
𝐹𝐹𝑓𝑓 2 𝐹𝐹𝑚𝑚𝑐𝑐𝐹𝐹𝑐𝑐𝑐𝑐𝐹𝐹𝑐𝑐𝑚𝑚𝑟𝑟 =
(6278 ∗ 2.889 + 6411 ∗ 2.773)
(2.889 + 2.773)
= 6343 𝑐𝑐𝑐𝑐 𝑐𝑐𝑚𝑚𝐹𝐹 µ 𝐹𝐹
CI (95%) — CI of mean concentration assumes that error between individual results has normal
distribution.
z is defined as 1.96. n = sample size (amount of replicates).
𝐶𝐶𝐶𝐶 𝑓𝑓𝐹𝐹𝐹𝐹 𝐹𝐹𝑚𝑚𝑐𝑐𝐹𝐹𝑐𝑐𝑐𝑐𝐹𝐹𝑐𝑐𝑚𝑚𝑟𝑟 = 𝑆𝑆𝑐𝑐𝐹𝐹𝑚𝑚𝑆𝑆𝐹𝐹𝐹𝐹𝑆𝑆 𝑆𝑆𝑚𝑚𝑣𝑣𝑐𝑐𝐹𝐹𝑐𝑐𝑐𝑐𝐹𝐹𝑚𝑚 ∗ 𝑧𝑧 ÷ √
𝑚𝑚
𝑀𝑀𝑚𝑚𝐹𝐹𝑚𝑚 𝑐𝑐𝐹𝐹𝑚𝑚𝑐𝑐𝑚𝑚𝑚𝑚𝑐𝑐𝐹𝐹𝐹𝐹𝑐𝑐𝑐𝑐𝐹𝐹𝑚𝑚 𝐹𝐹𝑓𝑓 𝐹𝐹𝑚𝑚𝑐𝑐𝐹𝐹𝑐𝑐𝑐𝑐𝐹𝐹𝑐𝑐𝑚𝑚𝑟𝑟