Qiagen QIAcuity 911000, User Manual

Page: 196 / 314

QIAcuity User Manual 06/2022 196
Status Code
Poisson test – linear distribution issue Blistering (Poisson test) 1
Poisson test areas of too low/no positive partitions amplification) 2
Poisson test areas of too high positive partitions (Proportion test – over-amplification) 3 & 4
Reference partition not filled 5 & 9
Dust detected by different reasons 6, 7, 8
Artifacts founds B & C

IsPositive – information whenever a partition was marked as positive (1) or negative (0).
For invalid partitions there is no information presented

RFU – RFU values associated with partition. For invalid partitions there is no information
present
7. Data will be exported to separate CSV files (one for each selected channel) and compressed
into one zip file.
Note : Please be informed that due to some corrections provided by analysis models,
sometimes there might be changes when comparing IsPositive column values with information
obtained by comparing RFU values to threshold.
RFU values (compact)
This option is very similar to regular RFU report but redundant data in columns: Well, Sample,
Channel, and Cycled volume in CSV file are removed to decrease the file size — same names or
values in subsequent rows will only be shown once until the name of value has changed.
Replicates
Replicates of a sample are identified by having the same reaction mix and the same sample name.
Replicates are detected automatically when the user assigns a sample to a well. If there is another
well with the same sample name and reaction mix, this well is identified as a Replicate.