Qiagen QIAcuity 911000, User Manual

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QIAcuity User Manual 06/2022 238
For tests having the configured targets in different wells/reaction mixes, the mean result is
calculated and shown for replicates in the selection. The list view is extended by 4 columns
indicating following mean values:

Mean concentration value

CI (95%) − CI of mean concentration as percentage

Mean fold change

CI (95%) − CI of mean fold change as percentage

Mean fold regulation
Note : If the selected analysis source contains replicates of the reference sample, your results can be
calculated only by using mean results for replicates. In this case the “Show mean values for
replicates” check box is marked and can’t be changed. In this case there is a warning message
above the table and when the user hover on the checkbox, a tooltip appears to inform the user that
selected analysis source contains replicates of the reference sample and result is calculated only by
using mean results for replicates.
To export the results to an CSV file, click Export to CSV .
Heatmap for gene expression
The Heatmap tab contains a heatmap view that shows the fold change in each of the wells. If a
well is not selected as a source for the analysis, the value is not displayed on the heatmap and its
background color is gray. In case that a fold change is not applicable for a well,
n.a. is shown.
To view an additional toolbar that enables actions related to the diagram, such as downloading
the plot, hold the pointer over the diagram. For more details about the toolbar, refer to Diagram
options.
To view detailed information about a particular well, point the cursor over the well. A tooltip with
detailed information opens.
To view the mean values for replicates in multiplex tests, click Show mean values for replicates . For
simplex tests the mean values view is always shown for replicates and cannot be disabled
Note : If the selected analysis source contains replicates of the reference sample, your results can be
calculated only by using mean results for replicates. In this case the “Show mean values for
replicates” checkbox is marked and can’t be changed. When the user hover on the checkbox, a