Qiagen QIAcuity 911000, User Manual

Page: 233 / 314

QIAcuity User Manual 06/2022 233
Point diagram tab for copy number variation
The Point diagram tab shows the diagram that displays the number of copies per genome of the
configured copy number variation test and the confidence intervals related to every value. A point
diagram has two axes. The x-axis shows the analyzed wells and samples, and the y-axis represents
the number of copies in each genome. A confidence interval displayed as an error bar is shown
for each of the points on the diagram.
To view an additional toolbar that enables actions related to the diagram, such as downloading
the plot, hold the pointer over the diagram. For more details about the toolbar, refer to Diagram
options
.
The y-axis scale can be modified using the buttons located below each graph. The buttons are
visible when you hold the pointer over a graph. To view the values on a linear scale, click
lin . To
view the values on a logarithmic scale, click log10 .
The diagram shows the values as points with the CI as interval for the selected samples. In case that
targets are in the same reaction mix, each sample is represented by 1 point. The point color reflects
the color assigned to the corresponding target of interest.
The samples are sorted by sample ID but the reference sample of a test is always shown. The well
IDs and sample IDs are labeled on the x-axis.
In case that targets are in different reaction mixes each combination of target of interest and
reference targets in one sample throughout the selected wells is shown in the diagram with a point
for the result. To view detailed information, hold the mouse pointer over its corresponding point. A
tooltip with detailed information opens.
To view the mean values for replicates, click Show mean values for replicates . If the user clicks into
the checkbox to select the mean representation for replicates, the points of individual replicates
disappear and only one point is shown at the sample label that represents the mean copies per
genome value of the replicates. When there are no replicates within selected wells the points don't
change. The corresponding well IDs of the replicates are shown on the x-axis. This is also applied
in same way by default to the view where targets are in different reaction mixes. In this case
individual replicate results are not available. Mean values are calculated and shown over all
replicates that are included in the well selection only. If there are further replicates of the same
sample which are not included in well selection, they are not considered in the calculated mean
value and show their individual results values.