Qiagen QIAcuity 911000, User Manual

Page: 177 / 314

QIAcuity User Manual 06/2022 177
Reasons for the invalidation of an image are:

Too less fluorescence signal, for example, when the Nanoplate was re-imaged after a long
storage period (see Operating Plates for information about the storage of plates).

Vibration during the imaging process can lead to blurry images. If the image of the reference
channel is affected, the number of valid partitions cannot be determined and the whole well is
invalidated for analysis. If a target channel is affected, only the image of the respective
channel is invalidated for analysis.

Incomplete filling of a well can lead to too less valid partitions in the reference channel
needed for analysis. In this case, the whole well is invalidated.
Image corrective measures
To ensure proper analysis based on valid partitions, artefacts that could influence the result analysis
are removed from the images. The corrections are done automatically by the QIAcuity Software
Suite and don’t require any user action. The partitions that are affected by artefacts are blacked
out and are invalidated for further analysis. Artefacts can be:

Dust and other particles

Low amplification areas

Areas of bad filling
Dust and other particles
Dust and other particles like hairs or strands are detected by the QIAcuity Software Suite and are
removed from the images. This figure shows an example of a well before and after dust/other
particles correction.