Qiagen QIAcuity 911000, User Manual

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QIAcuity User Manual 06/2022 241
In case that targets are in different reaction mixes each combination of target of interest and
reference targets in one sample throughout the selected wells is shown in the diagram with a point
for the result. To view detailed information, hold the mouse pointer over its corresponding point. A
tooltip with detailed information opens.
To view the mean values for replicates, click Show mean values for replicates. If the user clicks into
the checkbox to select the mean representation for replicates, the points of individual replicates
disappear and only one point is shown at the sample label that represents the mean fold change
value of the replicates. When there are no replicates within selected wells the points don't change.
The corresponding well IDs of the replicates are shown on the x-axis. This is also applied in same
way by default to the view where targets are in different reaction mixes. In this case individual
replicate results are not available. Mean values are calculated and shown over all replicates that
are included in the well selection only. If there are further replicates of the same sample which are
not included in well selection, they are not considered in the calculated mean value and show their
individual results values.
Note : If the selected analysis source contains replicates of the reference sample, your results can be
calculated only by using mean results for replicates. In this case the “Show mean values for
replicates” check box is marked and can’t be changed. In this case there is a warning message
above the diagram title and when the user hover on the checkbox, a tooltip appears to inform the
user that selected analysis source contains replicates of the reference sample and result is calculated
only by using mean results for replicates.
To add any of the point diagrams to the report, click Add to report next to the corresponding
diagram. For more information on reports, see section Reports.