Qiagen QIAcuity 911000, User Manual

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QIAcuity User Manual 06/2022 195
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Categories – names of channels
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Group – identifier of channels from the Categories column, which are positive (+) and
negative (−)
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Count – number of valid partitions in a certain group
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Total – total number of valid partitions in a well
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Volume – cycled volume in a well (in microliters). Value is corrected by VPF if it is applied.
Table 16. Example for a multiple occupancy export file
Well Hyperwell Categories Group Count Total Volume
A1 HW1 Green-Yellow-Orange-Red-Crimson +++++ 2000 8000 2.89
A1 HW1 Green-Yellow-Orange-Red-Crimson +++-- 200 8000 2.89
Note : The amount of valid partitions may differ from list view because, for multi-occupancy output,
only valid partitions across all selected channels are used as valid, whereas in list view, valid
partitions are defined per channel.
RFU values
If you want to export relative fluorescence units per partitions for selected wells, please follow the
instructions below.
1. Select wells that you want to get results for.
2. Click on the Channels tab, and select channels you are interested in.
3. Click on the Show results button.
4. In the List tab, above the table, on the right-hand side you can see the Export to CSV... option.
5. Click on the Export to CSV option and select the RFU values option.
6. Remember that data in CSV are structured as follows:
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Well – identifier of well
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Sample – identifier of sample
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Channel – first letter of channel on which picture was taken
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Cycled volume (µl) – well volume corrected by VPF

Threshold – threshold value

Partition – number by which a partition can be identified

Is Invalid – code for invalidation reason (check mapping table code statuses, there can be
a maximum 3 codes separated by a comma)