Qiagen QIAcuity 911000, User Manual

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QIAcuity User Manual 06/2022 255
If a plate upgrade fails, the following message is displayed. The not-upgraded plate is not changed
and shows the analysis results of the previous Suite version.
Volume Precision Factor (VPF)
The volume precision factor (VPF) offers a unique feature to secure consistency in precision of
concentration results obtained from a QIAcuity dPCR run. In general, Nanoplates provide partitions
of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation
of partition sizes in Nanoplate batches, caused by different stampers (molding form for
microstructures), can be addressed by applying the stamper-specific VPF. The VPF specifies the
exact cycled volume of a well within a Nanoplate and therefore further increases precision of
concentration calculation in each well. If a VPF is not available for a Nanoplate batch,
concentrations are calculated using an average cycled volume per plate until the VPF is imported.
The microstructure molding form is defined by the first two digits of the plate barcode.
Note : Multiple plate batches can come from one microstructure molding form.
New sets of VPFs will be published during production of the Nanoplate batches and can be
downloaded from the QIAGEN website.
Identification of plates without VPF
Plates without VPF can be analyzed using an average volume per plate. Such plates are marked
with the VPF (target) icon on the Plates Overview screen.