Qiagen QIAcuity 911000, User Manual

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QIAcuity User Manual 06/2022 239
tooltip appears to inform the user that selected analysis source contains replicates of the reference
sample and result is calculated only by using mean results for replicates.
To add the heatmap to the report, click Add to report . For more information on reports, see section
Reports.
Point diagram for gene expression
The Point diagram tab shows a point diagram view that displays the fold change values of
configured gene expression test and the confidence intervals related to every value. A point diagram
has two axes. The x-axis shows the analyzed wells and samples, and the y-axis represents the fold
change. A confidence interval displayed as an error bar is shown for each of the points on the
diagram.
To view an additional toolbar that enables actions related to the diagram, such as downloading
the plot, hold the pointer over the diagram. For more details about the toolbar, refer to Diagram
options.
The y-axis scale can be modified using the buttons located below each graph. The buttons are
visible when you hold the pointer over a graph. To view the values on a linear scale, click lin. To
view the values on a logarithmic scale, click log10.
The diagram shows the values as points with the CI as interval for the selected samples. In case that
the targets are in the same reaction mix, each sample is represented by 1 point. The point color
reflects the color assigned to the corresponding target of interest.
The samples are sorted by sample ID but the reference sample of a test is always shown first. The
well IDs and sample IDs are labeled on the x-axis.