Qiagen QIAcuity 911000, User Manual

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QIAcuity User Manual 06/2022 218
To add the heatmap diagram to the report, click Add to report. For more information on reports,
see section Reports.
Point diagram tab for mutation detection
The Point diagram tab shows a diagram that displays the percentage of mutant fractions in each
analyzed well. A point diagram has two axes. The x-axis shows the analyzed wells and samples,
and the y-axis represents the mutant fraction, shown as a percentage.
To view an additional toolbar that enables actions related to the diagram, such as downloading
the plot, hold the pointer over the diagram. For more details about the toolbar, refer to section
Diagram options.
The y-axis scale can be modified from linear to logarithmic scale using the buttons located on the
left below the diagram. The buttons are visible when you hold the pointer over a graph. To view
the values on a linear scale, click
lin . To view the values on a logarithmic scale, click log10 .
Each combination of wild type target and mutant target in one sample throughout the selected wells
is represented in the diagram with one point showing the concentration value together with the
confidence interval. To view detailed information, hold the mouse pointer over its corresponding
point. A tooltip with detailed information opens.
To view the mean values for replicates for multiplex test, click Show mean values for replicates. If
the user clicks into the checkbox to select the mean representation for replicates, the points of
individual replicates disappear and only one point is shown at the sample label that represents the
mean mutant fraction value of the replicates. When there are no replicates within selected wells for
mutant target the points don't change. The corresponding well IDs of the replicates are shown on