Qiagen QIAcuity 911000, User Manual

Page: 258 / 314

QIAcuity User Manual 06/2022 258
Operating Plates
In the QIAcuity plate-based system, one reaction mix per well is partitioned into a large number of
individual partitions prior to the amplification step, resulting in one or very few templates being
present in each partition. QIAGEN offers three different plate types according to specific user
needs.
Table 19. Plate types according to user needs
Plate type Frame color No. of wells Input volume/well ( µl ) No. of partitions Partition volume ( nl)
Nanoplate 26K
24-well
Blue 24 40 Approx. 26000 Approx. 0.82
Nanoplate 26K
8-well
Light blue 8 40 Approx. 26000 Approx. 0.82
Nanoplate 8.5K
24-well
White 24 12 Approx. 8500 Approx. 0.34
Nanoplate 8.5K
96-well
Gray 96 12 Approx. 8500 Approx. 0.34
Please note that the QIAcuity Software Suite calculates with a partition volume of 0.82 nl and
0.34 nl in case the VPF (volume precision factor) has not been applied. If the VPF has been loaded
to the software, the volume of each well is Nanoplate batch–specific calibrated and used for
concentration calculation. Thus, the concentration calculated by the QIAcuity Software Suite will
differ to concentration values calculated manually.
Image of Nanoplate 26K 24-well, Nanoplate 8.5K 24-well, and Nanoplate 8.5K 96-well.