FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
92
0112-0127 B
Triturate Selection
Trituration is mixing of the contents of the wells in either or both the
compound source and assay plates. Trituration is accomplished by fluid
being alternately aspirated from and dispensed back into a well.
Applications for which trituration is recommended include when
compound in the source plate needs to be resuspended before addition,
transferring low volumes to the read plate, or when transferring fluid
before or during an absorbance read to ensure that there is proper
mixing of the well contents.
Select the transfer (if more than one is enabled) during which you wish to
perform trituration, and then select the check box for either
Compound
Source
or
Assay Plate
, or both.
You can set the
Volume
of fluid to be withdrawn from the well and the
number of times (Cycles) to be aspirated and dispensed into that well.
In addition, for assay plates, you must also enter a value for the
Height
at
which the trituration occurs. The height setting should take into account
the volume selected, so that the tips remain below the liquid surface and
do not draw air.
Figure 3-25:
Triturate Settings
If you choose dispense times that are incompatible with the settings, an
error message appears.
Figure 3-26:
Triturate Error Message
To modify the dispensing time, return to the Transfer Settings in the
Compound Transfer settings.
Note:
The choices shown in this section are based on the number of
compound transfers you specified in the Compound Transfer settings. If
no transfers are enabled, no triturate settings are applicable.
FlexStation_manual.book Page 92 Tuesday, June 25, 2013 10:22 AM
