FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
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0112-0127 B
Wavelengths
Up to four wavelengths can be read per well. Although the arrows open a
window showing the six most commonly-used wavelengths, the tunable
monochromator allows any excitation or emission wavelength to be
entered between a certain range for the particular read mode. In
addition to an emission monochromator, fluorescent-quality cutoff filters
help reduce background signal.
You can either manually choose from 15 emission filters or allow the
software to choose which long pass emission filter to use based on the
excitation wavelength. See
Table 3-4: Emission Cutoff Filter Default
Sensitivity
Adjust sensitivity either by varying the number of readings that are
performed in each well or by changing the PMT sensitivity (voltage). In
general, a higher number of readings results in better reader precision. In
fluorescence intensity reading, the sample can photobleach if a high
number of flashes are used, so if the plate will be re-read (especially for
kinetic assays or multiwavelength reads), Molecular Devices
recommends using 10 readings or fewer. During Flex mode, Molecular
Devices recommends that you begin with 6 readings to supply enough
light for these fast kinetic assays. For low-signal endpoint assays, such as
some FP or TRF assays, 100 readings per well can be optimal.
The Automatic PMT sensitivity setting allows the reader to adjust the
PMT voltage automatically for varying signals coming from samples on
the plate. In modes that allow manual setting of the PMT, a setting of
High is best for low signal assays, including assays that require Flex mode.
The PMT sensitivity is not adjustable for all read types, including Kinetic
and Flex reads.
Figure 3-14:
Sensitivity Settings
Note:
Increasing the number of reads also increases the total read
time.
FlexStation_manual.book Page 80 Tuesday, June 25, 2013 10:22 AM
