Performance Specifications
0112-0127 B
157
Table B-4:
Fluorescence Intensity Performance
Item
Specification
Sensitivity Top
Read
< 5 pM FITC, 1 fmol/200
μ
L (96)
< 20 pM, 2 fmol/100
μ
L (384)
Wavelength range
250 nm to 850 nm
Wavelength selection
Monochromators, tunable in 1 nm increments
Bandwidth (excitation,
emission)
9 nm, 15 nm
Number of
excitation/emission pairs
per plate
4
Dynamic range
106 in 96-well black plates: auto gain circuitry
System validation
Self-calibrating with built-in fluorescence calibrators
Light source
Xenon flash lamp (1 joule/flash)
Average lamp lifetime
2 years normal operation
Detector
Photomultiplier
Table B-5:
Fluorescence Polarization Performance
Item
Specification
Wavelength range
400 nm to 750 nm
Wavelength selection
Monochromators, tunable in 1 nm increments
Bandwidth (excitation,
emission)
9 nm, 15 nm
Precision
< 5 mP standard deviation at 1 nM fluorescein
in 96-well and 384-well microplates
FlexStation_manual.book Page 157 Tuesday, June 25, 2013 10:22 AM
