FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
40
0112-0127 B
Endpoint Reads
In Endpoint reads, as well as in Kinetic and Flex reads, you can select
from one to four excitation/emission pairs to obtain readings for each
well of a microplate.
In Endpoint reads, one to eight or one to sixteen wells in one column of
the assay plate are delivered before starting the read. At a preselected
point or points before the read, the pipettor can transfer up to three
reagents from the compound plate to the assay plate. After reagents are
transferred, the read initiates for the entire read area. Unlike Flex, the
read area is not limited to one column at a time in an Endpoint read. All
the data are collected in one data file represented as a 96-well or
384-well microplate.
For more information on this read type, please review the appropriate
section in the SoftMax Pro user guide.
Kinetic Reads
Kinetic analysis can be performed for a total run time of up to 99 hours.
The kinetic read interval depends upon the instrument setup parameters
selected in the SoftMax Pro Software, but is limited to 2 hours and 45
minutes (165 minutes). At the end of a reading, rates are reported as
each well. Kinetic analysis has many advantages when determining the
relative activity of an enzyme in different types of assays, including the
purification and characterization of enzymes and enzyme conjugates.
In Kinetic reads, one to eight or one to sixteen wells in one column of the
assay plate can be delivered before starting the read. At a preselected
point or points before the read, the pipettor can transfer up to three
reagents from the compound plate to the assay plate. After reagents are
transferred, the read initiates for the entire read area. Unlike Flex, the
read area is not limited to one column at a time in Kinetic reads. All the
data are collected in one data file represented as a 96-well or 384-well
microplate.
For more information on this read type, please review the appropriate
section in the SoftMax Pro user guide.
Assay plate
type
Yes
Yes
Yes
Yes
Yes
Compound
plate type
Yes
Yes
No
No
Yes
Table 1-1:
Operation Modes and Features (cont’d)
Operation
Modes
Endpoint
Kinetic
Spectrum
Well Scan
Flex
FlexStation_manual.book Page 40 Tuesday, June 25, 2013 10:22 AM
