Glossary
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Kinetic
In a Kinetic read, the instrument collects data over time with multiple
readings taken at regular intervals. To achieve the shortest possible
interval for Kinetic readings, choose wavelengths in ascending order.
The values calculated based on raw kinetic data include VMax, VMax per
Sec, Time to VMax, and Onset Time. Kinetic readings can be single-
wavelength or multiple-wavelength readings.
Luminescence
Luminescence is the emission of light by processes that derive energy
from essentially non-thermal changes, the motion of subatomic particles,
or the excitation of an atomic system by radiation. Luminescence
detection relies on the production of light from a chemical reaction in a
sample.
Photomultiplier Tube (PMT)
A Photomultiplier Tube (PMT) is a vacuum tube that detects light from
dim sources through the use of photoemission and successive instances
of secondary emission to produce enough electrons to generate a useful
current.
Read Mode
The read mode is type of reading performed: absorbance, fluorescence
intensity, time-resolved fluorescence, fluorescence polarization, or
luminescence.
Read Type
The read type is the method used to read the microplate: endpoint,
kinetic, spectrum, or well scan.
Readings per Well
The reading per well is the user-definable number of times that readings
are taken on a well in fluorescence mode or the amount of time that data
is collected using the luminescence read mode.
SoftMax Pro Software
The SoftMax Pro Software is an integrated software program from
Molecular Devices that is used to control and collect data from Molecular
Devices microplate readers.
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