FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
34
0112-0127 B
Mixing
Mixing can be accomplished in one of two ways, using either the
Trituration or Automix functions. Use of these functions are dependant
on the assay performed and the read mode used.
•
Trituration is mixing of the well contents in either the compound
or assay plates. This is accomplished by fluid being alternately
aspirated from and dispensed back into a well using the pipettor.
Trituration is available only during assays that include fluid
transfer, including Flex, Endpoint, or Kinetic modes, and can be
performed in either the compound or assay plate. Within the
compound plate, trituration can be used to resuspend
compounds that might have crashed out of solution.
Alternatively, it can be used to promote prompt mixing in the
assay plate when delivering small reagent volumes for fast
kinetic cell-based assays, such as calcium mobilization.
•
The Automix function permits automatic shaking of the
microplate at preset intervals, thereby mixing the contents
within each well. Automix must be selected before beginning a
reading. Automix settings vary with assay type.
For Endpoint assays, enabling Automix shakes the plate for a
definable number of seconds and then reads at all selected
wavelengths.
For Kinetic assays, two types of Automix can be enabled. You
can set Automix to shake the plate for a definable number of
seconds before the initial reading or for a definable number
of seconds before each subsequent reading.
Use of Automix is strongly recommended for ELlSA and other
solid-phase, enzyme-mediated reactions to enhance accuracy.
Note:
Trituration in the assay plate well may agitate cells,
causing responses not associated with the compound addition.
Assay development should be performed to determine if
trituration is necessary for the assay.
FlexStation_manual.book Page 34 Tuesday, June 25, 2013 10:22 AM
